Difference between revisions of "Rapp"
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− | + | {| style="text-align: left" | |
− | + | ! Summary: | |
− | + | | Rapp Phototargeting Unit | |
− | + | |- valign="top" | |
− | + | ! Author: | |
− | + | | Arthur Edelstein | |
+ | |- | ||
+ | ! License: | ||
+ | | LGPL | ||
+ | |- | ||
+ | ! Platforms: | ||
+ | | Windows | ||
+ | |- valign="top" | ||
+ | ! Devices: | ||
+ | | Rapp UGA-40 | ||
+ | |} | ||
+ | ---- | ||
The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager [[Projector Plugin]] allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition). | The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager [[Projector Plugin]] allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition). | ||
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A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include: | A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include: | ||
− | + | {| class="wikitable" | |
− | + | ! Property | |
− | + | ! Description | |
− | + | |- | |
− | + | ! CalibrationMode | |
− | + | | If "On", laser is turned on regardless of targeting status | |
− | + | |- | |
− | + | ! TTLTriggered | |
− | + | | For TTL triggering, determines whether triggering occurs on a rising or falling edge | |
+ | |- | ||
+ | ! SpotSize | ||
+ | | Tells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs | ||
+ | |- | ||
+ | ! RasterFrequency_Hz | ||
+ | | Controls the speed of ROI rastering | ||
+ | |- | ||
+ | ! AccuracyPercent | ||
+ | | Controls how precisely an irregular ROI is filled in during rastering | ||
+ | |- | ||
+ | ! OnMinimumRectSize | ||
+ | | Determines the smallest tile used during rastering | ||
+ | |- | ||
+ | ! Sequence | ||
+ | | Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used) | ||
+ | |} | ||
Revision as of 06:15, 27 October 2013
Summary: | Rapp Phototargeting Unit |
---|---|
Author: | Arthur Edelstein |
License: | LGPL |
Platforms: | Windows |
Devices: | Rapp UGA-40 |
The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager Projector Plugin allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition).
The UGA-40 supports up to two lasers of different colors. Choose which laser to use by setting the "Laser" property to "1" or "2".
A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include:
Property | Description |
---|---|
CalibrationMode | If "On", laser is turned on regardless of targeting status |
TTLTriggered | For TTL triggering, determines whether triggering occurs on a rising or falling edge |
SpotSize | Tells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs |
RasterFrequency_Hz | Controls the speed of ROI rastering |
AccuracyPercent | Controls how precisely an irregular ROI is filled in during rastering |
OnMinimumRectSize | Determines the smallest tile used during rastering |
Sequence | Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used) |
Resources
Click here to search for Rapp on the Micro-Manager mailing list archive.