Difference between revisions of "Rapp"
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| − | + | {| style="text-align: left" | |
| − | + | ! Summary: | |
| − | + | | Rapp Phototargeting Unit | |
| − | + | |- valign="top" | |
| − | + | ! Author: | |
| − | + | | Arthur Edelstein | |
| + | |- | ||
| + | ! License: | ||
| + | | LGPL | ||
| + | |- | ||
| + | ! Platforms: | ||
| + | | Windows | ||
| + | |- valign="top" | ||
| + | ! Devices: | ||
| + | | Rapp UGA-40 | ||
| + | |} | ||
| + | ---- | ||
The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager [[Projector Plugin]] allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition). | The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager [[Projector Plugin]] allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition). | ||
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A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include: | A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include: | ||
| − | + | {| class="wikitable" | |
| − | + | ! Property | |
| − | + | ! Description | |
| − | + | |- | |
| − | + | ! CalibrationMode | |
| − | + | | If "On", laser is turned on regardless of targeting status | |
| − | + | |- | |
| − | + | ! TTLTriggered | |
| − | + | | For TTL triggering, determines whether triggering occurs on a rising or falling edge | |
| + | |- | ||
| + | ! SpotSize | ||
| + | | Tells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs | ||
| + | |- | ||
| + | ! RasterFrequency_Hz | ||
| + | | Controls the speed of ROI rastering | ||
| + | |- | ||
| + | ! AccuracyPercent | ||
| + | | Controls how precisely an irregular ROI is filled in during rastering | ||
| + | |- | ||
| + | ! OnMinimumRectSize | ||
| + | | Determines the smallest tile used during rastering | ||
| + | |- | ||
| + | ! Sequence | ||
| + | | Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used) | ||
| + | |} | ||
Revision as of 07:15, 27 October 2013
| Summary: | Rapp Phototargeting Unit |
|---|---|
| Author: | Arthur Edelstein |
| License: | LGPL |
| Platforms: | Windows |
| Devices: | Rapp UGA-40 |
The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager Projector Plugin allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition).
The UGA-40 supports up to two lasers of different colors. Choose which laser to use by setting the "Laser" property to "1" or "2".
A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include:
| Property | Description |
|---|---|
| CalibrationMode | If "On", laser is turned on regardless of targeting status |
| TTLTriggered | For TTL triggering, determines whether triggering occurs on a rising or falling edge |
| SpotSize | Tells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs |
| RasterFrequency_Hz | Controls the speed of ROI rastering |
| AccuracyPercent | Controls how precisely an irregular ROI is filled in during rastering |
| OnMinimumRectSize | Determines the smallest tile used during rastering |
| Sequence | Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used) |
Resources
Click here to search for Rapp on the Micro-Manager mailing list archive.