Difference between revisions of "Rapp"
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<tr><td>'''Sequence'''</td><td>Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used)</td></tr> | <tr><td>'''Sequence'''</td><td>Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used)</td></tr> | ||
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Revision as of 06:08, 27 October 2013
| Summary: | Rapp UGA-40 Phototargeting Unit |
| Author: | Arthur Edelstein |
| License: | LGPL |
| Platform: | Windows |
The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager Projector Plugin allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition).
The UGA-40 supports up to two lasers of different colors. Choose which laser to use by setting the "Laser" property to "1" or "2".
A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include:
| CalibrationMode | If On, laser is turned on regardless of targeting status |
| TTLTriggered | For TTL triggering, determines whether triggering occurs on a rising or falling edge |
| SpotSize | Tells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs |
| RasterFrequency_Hz | Controls the speed of ROI rastering |
| AccuracyPercent | Controls how precisely an irregular ROI is filled in during rastering |
| OnMinimumRectSize | Determines the smallest tile used during rastering |
| Sequence | Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used) |
Resources
Click here to search for Rapp on the Micro-Manager mailing list archive.