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Difference between revisions of "Micro-Manager at the ASCB Meeting 2011"

(Created page with "For the fifth year we presented the Micro-Manager software in the Exhibit Hall at the annual meeting of the American Society for Cell Biology (ASCB) in Denver. Our goal was to in...")
 
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| <font size=-1>Drosophila S2 cell expressing GFP-histone and cherry-tubulin (from Eric Griffis). Images were taken during the ASCB meeting at 15 second intervals. At each time point, a z-stack of 5 slices (with 2 micron intervals) was acquired. Exposure time was 50 msec, total time to acquire a single time point (10 images) was less than 700 msec. </font>
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| <font size=-1>Drosophila S2 cell expressing gfp-histone h2B and cherry-tubulin (from Eric Griffis). Images were taken during the ASCB meeting at 15 second intervals. At each time point, a z-stack of 5 slices (with 2 micron intervals) was acquired. Exposure time was 50 msec, total time to acquire a single time point (10 images) was less than 700 msec. </font>
 
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We were very encouraged by the interest and overwhelmingly positive response both from the user community and from the industry. On the ASCB exhibit floor we noted Micro-Manager controlling equipments in the booths of Spectral, [http://www.raptorphotonics.com/search.php Raptor Photonics], [http://www.axiomoptics.com/index_Events.html Axiom Optics], [http://www.sutter.com/index.html Sutter Instrument], [http://www.thorlabs.us/Navigation.cfm?section=7 ThorLabs], and [http://biobus.org/ BioBus]. Thanks everyone for stopping by at our booth and for all the support our project is getting from the industry!
 
We were very encouraged by the interest and overwhelmingly positive response both from the user community and from the industry. On the ASCB exhibit floor we noted Micro-Manager controlling equipments in the booths of Spectral, [http://www.raptorphotonics.com/search.php Raptor Photonics], [http://www.axiomoptics.com/index_Events.html Axiom Optics], [http://www.sutter.com/index.html Sutter Instrument], [http://www.thorlabs.us/Navigation.cfm?section=7 ThorLabs], and [http://biobus.org/ BioBus]. Thanks everyone for stopping by at our booth and for all the support our project is getting from the industry!

Revision as of 17:10, 12 December 2011

For the fifth year we presented the Micro-Manager software in the Exhibit Hall at the annual meeting of the American Society for Cell Biology (ASCB) in Denver. Our goal was to introduce the Micro-Manager software to new users, gain feedback from current Micro-Manager users, and to demonstrate new capabilities.

To demonstrate the software we used a spinning disk confocal system (Yokogawa/Solamere) equipped with a Photometrics Evolve 512 camera, ASI XYZ and piezo Z-stage on the new ASI RAMM Microscope, multi-color laser launch and AOTF (Solamere), ESImaging micro-controller, and running on a PC computer. All of the equipment was made available to us by the respective companies (Thanks!).

For fast image acquisition, a triggering mechanism was set-up, resulting in continuous acquisition at a rate limited almost only by the exposure time. The triggering was made possible by synchronization of the camera, AOTF and z-piezo stage. A TTL pulse, which signals when the camera is exposing, was used to drive a sequence of different output patterns on the ESImaing micro-controller.

Drosophila S2 cell expressing gfp-histone h2B and cherry-tubulin (from Eric Griffis). Images were taken during the ASCB meeting at 15 second intervals. At each time point, a z-stack of 5 slices (with 2 micron intervals) was acquired. Exposure time was 50 msec, total time to acquire a single time point (10 images) was less than 700 msec.

We were very encouraged by the interest and overwhelmingly positive response both from the user community and from the industry. On the ASCB exhibit floor we noted Micro-Manager controlling equipments in the booths of Spectral, Raptor Photonics, Axiom Optics, Sutter Instrument, ThorLabs, and BioBus. Thanks everyone for stopping by at our booth and for all the support our project is getting from the industry!

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