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Localization Microscopy

Revision as of 10:30, 17 April 2012 by Ziah Dean (Talk | contribs)

Introduction

This plugin is primarily focused on the localization of single fluorescent molecules for either tracking or reconstruction of super-resolution images. This plugin has been carefully packaged to allow fast peak-fitting and rendering that works “out of the box”. A dialogue window allows users access to comprehensive list of options for fitting and tracking single molecules. A second window allows users to various options for data processing such as rendering, drift correction, etc.

Break down of Dialogue window

Image-Prams.JPG

Image Parameters:

Max-settings.JPG

Finding Maxima

Fit-prams.JPG

Fitting Parameters

Filter-data.JPG

Filtering Data

Data-processing.JPG
Data Processing
Fit-button.JPG blank
Track-button.JPG blank
MTrack-button.JPG MTrack: blank
Data-button.JPG Data: blank
Stop-button.JPG Stop: blank

Data processing window

LM DataProcWindow.png

Drift correction

2C ref. and cor.

Plotting

Rendering

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