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Difference between revisions of "Localization Microscopy"

(Introduction)
(Break down of Dialogue window)
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__NOTOC__
 
__NOTOC__
 
<table cellspacing=15><tr><td>
 
<table cellspacing=15><tr><td>
[[Image: LM_DialogueWindow.png]]</td>
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[[Image: Image-Prams.JPG]]</td>
 
<td>
 
<td>
====Image Parameters====
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====Image Parameters:====
 
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</td>
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</table>
  
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<table cellspacing=15><tr><td>
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[[Image: Max-settings.JPG]]</td>
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<td>
 
====Finding Maxima====
 
====Finding Maxima====
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</td>
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</table>
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<table cellspacing=15><tr><td>
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[[Image: Fit-prams.JPG]]</td>
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<td>
 
====Fitting Parameters====
 
====Fitting Parameters====
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</td>
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</table>
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<table cellspacing=15><tr><td>
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[[Image: Filter-data.JPG]]</td>
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<td>
 
====Filtering Data====
 
====Filtering Data====
 
</td>
 
</td>
</tr>
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</table>
</table>  
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<table cellspacing=15><tr><td>
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[[Image: Data-processing.JPG]]</td></tr>
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<td> '''Data Processing'''
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</td> <tr><td>
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{|
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|-
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|[[Image: Fit-button.JPG]]  blank
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|-
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|[[Image: Track-button.JPG]] blank
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|-
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|[[Image: MTrack-button.JPG]] MTrack: blank
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|-
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|[[Image: Data-button.JPG]] Data: blank
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|-
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|[[Image:Stop-button.JPG ]] Stop: blank
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|}
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</td></tr>
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</table>
  
 
==Data processing window==
 
==Data processing window==

Revision as of 10:28, 17 April 2012

Introduction

This plugin is primarily focused on the localization of single fluorescent molecules for either tracking or reconstruction of super-resolution images. The new super-resolution imaging techniques (PALM/STORM) have been shown to become highly useful for many researchers seeking new depths in their research. Similarly the advancements in single molecule tracking techniques have been used to determine behavior of single proteins. We aim to make these techniques readily available for researchers to have free and easy access. This plugin has been carefully packaged to allow fast peak-fitting and rendering that works “out of the box”. A dialogue window allows users access to comprehensive list of options for fitting and tracking single molecules. A second window allows users to various options for data processing such as rendering, drift correction, etc.

Break down of Dialogue window

Image-Prams.JPG

Image Parameters:

Max-settings.JPG

Finding Maxima

Fit-prams.JPG

Fitting Parameters

Filter-data.JPG

Filtering Data

Data-processing.JPG
Data Processing
Fit-button.JPG blank
Track-button.JPG blank
MTrack-button.JPG MTrack: blank
Data-button.JPG Data: blank
Stop-button.JPG Stop: blank

Data processing window

LM DataProcWindow.png

Drift correction

2C ref. and cor.

Plotting

Rendering

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