Papers citing Micro-Manager

Papers citing and/or acknowledging the use of Micro-Manager

2015

Bump, S. (2015). Microscopic characterization of functionalized paper as a platform for 3D cell cultures. Ph.D. Thesis. Technische Universität.

Lin, S., Barrette, A.M., Chapin, C., Gonzales, L.W., Gonzalez, R.F., Dobbs, L.G., and Ballard, P.L. (2015). Expression of human carcinoembryonic antigen‐related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. Physiological Reports 3, e12657 <doi:10.14814%2Fphy2.12657>.

Sevenler, D., and Selim Ünlü, M. (2015). Numerical techniques for high-throughput reflectance interference biosensing. Journal of Modern Optics 1–6 <doi:10.1080%2F09500340.2015.1117668>.

Morozova, N., Sabantsev, A., Bogdanova, E., Fedorova, Y., Maikova, A., Vedyaykin, A., Rodic, A., Djordjevic, M., Khodorkovskii, M., and Severinov, K. (2015). Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system. Nucl. Acids Res. gkv1490 <doi:10.1093%2Fnar%2Fgkv1490>.

Will, C., Messlinger, K., and Fischer, M.J.M. (2016). Vessel diameter measurements at the medullary brainstem in vivo as an index of trigeminal activity. Brain Research 1632, 51–57 <doi:10.1016%2Fj.brainres.2015.12.013>.

Bazin, D., Jouanneau, C., Bertazzo, S., Sandt, C., Dessombz, A., Réfrégiers, M., Dumas, P., Frederick, J., Haymann, J.-P., Letavernier, E., et al. Combining field effect scanning electron microscopy, deep UV fluorescence, Raman, classical and synchrotron radiation Fourier transform Infra-Red Spectroscopy in the study of crystal-containing kidney biopsies. Comptes Rendus Chimie <doi:10.1016%2Fj.crci.2015.03.001>.

Urban, N., Wang, L., Kwiek, S., Rademann, J., Kuebler, W.M., and Schaefer, M. (2016). Identification and Validation of Larixyl Acetate as a Potent TRPC6 Inhibitor. Mol Pharmacol 89, 197–213 <doi:10.1124%2Fmol.115.100792> .

Cinquin, B., Maigre, L., Pinet, E., Chevalier, J., Stavenger, R.A., Mills, S., Réfrégiers, M., and Pagès, J.-M. (2015). Microspectrometric insights on the uptake of antibiotics at the single bacterial cell level. Scientific Reports 5, 17968 <doi:10.1038%2Fsrep17968>.

Yun, K., Lee, H., Bang, H., and Li Jeon, N. (2015). QR-on-a-chip: Computer-recognizable micro-pattern engraved microfluidic device for high-throughput image acquisition. Lab on a Chip <doi:10.1039%2FC5LC01350J>.

Dudek, M., Gossan, N., Yang, N., Im, H.-J., Ruckshanthi, J.P.D., Yoshitane, H., Li, X., Jin, D., Wang, P., Boudiffa, M., et al. (2015). The chondrocyte clock gene Bmal1 controls cartilage homeostasis and integrity. J Clin Invest 126, 0–0 <doi:10.1172%2FJCI82755>.

Smith, A.J., and Verkman, A.S. (2015). Superresolution Imaging of Aquaporin-4 Cluster Size in Antibody-Stained Paraffin Brain Sections. Biophysical Journal 109, 2511–2522 <doi:10.1016%2Fj.bpj.2015.10.047>.

Lee, C., Roberts, S.E., and Gladfelter, A.S. Quantitative spatial analysis of transcripts in multinucleate cells using single-molecule FISH. Methods <doi:10.1016%2Fj.ymeth.2015.12.007>.

Handly, L.N., Pilko, A., and Wollman, R. (2015). Paracrine communication maximizes cellular response fidelity in wound signaling. eLife Sciences 4, e09652 <doi:10.7554%2FeLife.09652>.

Pattanayak, G.K., Lambert, G., Bernat, K., and Rust, M.J. (2015). Controlling the Cyanobacterial Clock by Synthetically Rewiring Metabolism. Cell Reports 13, 2362–2367 <doi:10.1016%2Fj.celrep.2015.11.031>.

Kath, J.E., Chang, S., Scotland, M.K., Wilbertz, J.H., Jergic, S., Dixon, N.E., Sutton, M.D., and Loparo, J.J. (2015). Exchange between Escherichia coli polymerases II and III on a processivity clamp. Nucl. Acids Res. gkv1375 <doi:10.1093%2Fnar%2Fgkv1375>.

Croft, W., Reusch, K., Tilunaite, A., Russell, N.A., Thul, R., and Bellamy, T.C. (2015). Probabilistic encoding of stimulus strength in astrocyte global calcium signals. Glia n/a – n/a <doi:10.1002%2Fglia.22947>.

Day, C.R., Chen, H., Coulon, A., Meier, J.L., and Larson, D.R. High-throughput single-molecule screen for small-molecule perturbation of splicing and transcription kinetics. Methods <doi:10.1016%2Fj.ymeth.2015.11.025>.

Stephen, T.-L., Higgs, N.F., Sheehan, D.F., Awabdh, S.A., López-Doménech, G., Arancibia-Carcamo, I.L., and Kittler, J.T. (2015). Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling. J. Neurosci. 35, 15996–16011 <doi:10.1523%2FJNEUROSCI.2068-15.2015>.

Gu, X., Li, A., Liu, S., Lin, L., Xu, S., Zhang, P., Li, S., Li, X., Tian, B., Zhu, X., et al. (2015). MicroRNA124 Regulated Neurite Elongation by Targeting OSBP. Mol Neurobiol 1–9 <doi:10.1007%2Fs12035-015-9540-4>.

Zhao, Z., Yang, Y., Zeng, Y., and He, M. (2016). A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis. Lab on a Chip <doi:10.1039%2FC5LC01117E>.

Smith, G.B., Whitney, D.E., and Fitzpatrick, D. (2015). Modular Representation of Luminance Polarity in the Superficial Layers of Primary Visual Cortex. Neuron 88, 805–818 <doi:10.1016%2Fj.neuron.2015.10.019>.

Splitt, S.D., and Risser, D.D. (2015). The non-metabolizable sucrose analog sucralose is a potent inhibitor of hormogonium differentiation in the filamentous cyanobacterium Nostoc punctiforme. Arch Microbiol 1–11 <doi:10.1007%2Fs00203-015-1171-7>.

Lanier, M.H., McConnell, P., and Cooper, J.A. (2015). Cell Migration and Invadopodia Formation Require a Membrane-binding Domain of CARMIL2. J. Biol. Chem. jbc.M115.676882 <doi:10.1074%2Fjbc.M115.676882>.

Smith, G.B., Whitney, D.E., and Fitzpatrick, D. (2015). Modular Representation of Luminance Polarity in the Superficial Layers of Primary Visual Cortex. Neuron 88, 805–818 <doi:10.1016%2Fj.neuron.2015.10.019>.

Shigeto, M., Ramracheya, R., Tarasov, A.I., Cha, C.Y., Chibalina, M.V., Hastoy, B., Philippaert, K., Reinbothe, T., Rorsman, N., Salehi, A., et al. (2015). GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clin Invest 125, 4714–4728 <doi:10.1172%2FJCI81975>.

Shekhar, S., Kerleau, M., Kühn, S., Pernier, J., Romet-Lemonne, G., Jégou, A., and Carlier, M.-F. (2015). Formin and capping protein together embrace the actin filament in a menage a trois. Nat Commun 6, 8730 <doi:10.1038%2Fncomms9730>.

Ripple, D.C., and Hu, Z. (2015). Correcting the Relative Bias of Light Obscuration and Flow Imaging Particle Counters. Pharm Res 1–20 <doi:10.1007%2Fs11095-015-1817-9>.

Rost, B.R., Schneider, F., Grauel, M.K., Wozny, C., Bentz, C.G., Blessing, A., Rosenmund, T., Jentsch, T.J., Schmitz, D., Hegemann, P., et al. (2015). Optogenetic acidification of synaptic vesicles and lysosomes. Nat Neurosci 18, 1845–1852 <doi:10.1038%2Fnn.4161>.

Hersey, J.S., Meller, A., and Grinstaff, M.W. (2015). Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal. Chem. 87, 11863–11870 <doi:10.1021%2Facs.analchem.5b03386>.

Nosrati, R., Driouchi, A., Yip, C.M., and Sinton, D. (2015). Two-dimensional slither swimming of sperm within a micrometre of a surface. Nat Commun 6, 8703 <doi:10.1038%2Fncomms9703>.

Hoera, C., Skadell, M.M., Pfeiffer, S.A., Pahl, M., Shu, Z., Beckert, E., and Belder, D. (2016). A chip-integrated highly variable thermal flow rate sensor. Sensors and Actuators B: Chemical 225, 42–49 <doi:10.1016%2Fj.snb.2015.11.009>.

Huang, H., Alvarez, S., Bindbeutel, R.K., Shen, Z., Naldrett, M.J., Evans, B.S., Briggs, S.P., Hicks, L.M., Kay, S.A., and Nusinow, D.A. (2015). Identification of evening complex associated proteins in Arabidopsis by affinity purification and mass spectrometry. Mol Cell Proteomics mcp.M115.054064 <doi:10.1074%2Fmcp.M115.054064>.

Lenstra, T.L., Coulon, A., Chow, C.C., and Larson, D.R. (2015). Single-Molecule Imaging Reveals a Switch between Spurious and Functional ncRNA Transcription. Molecular Cell 60, 597–610 <doi:10.1016%2Fj.molcel.2015.09.028>.

Krieger, J.W., Singh, A.P., Bag, N., Garbe, C.S., Saunders, T.E., Langowski, J., and Wohland, T. (2015). Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms. Nat. Protocols 10, 1948–1974 <doi:10.1038%2Fnprot.2015.100>.

Acerbi, I., Cassereau, L., Dean, I., Shi, Q., Au, A., Park, C., Chen, Y.Y., Liphardt, J., Hwang, E.S., and Weaver, V.M. (2015). Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration. Integr. Biol. 7, 1120–1134 <doi:10.1039%2FC5IB00040H>.

Romsey, N.R., Hou, Y., Rodriguez, L.L., and Schneider, I.C. (2013). The Number of Lines a Cell Contacts and Cell Contractility Drive the Efficiency of Contact Guidance. Cel. Mol. Bioeng. 7, 122–135 <doi:10.1007%2Fs12195-013-0299-2>.

Prevo, B., Mangeol, P., Oswald, F., Scholey, J.M., and Peterman, E.J.G. (2015). Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia. Nat Cell Biol 17, 1536–1545 <doi:10.1038%2Fncb3263>.

Treweek, J.B., Chan, K.Y., Flytzanis, N.C., Yang, B., Deverman, B.E., Greenbaum, A., Lignell, A., Xiao, C., Cai, L., Ladinsky, M.S., et al. (2015). Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping. Nat. Protocols 10, 1860–1896 <doi:10.1038%2Fnprot.2015.122>.

Jönsson, P., and Jönsson, B. (2015). Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows. Langmuir 31, 12708–12718 <doi:10.1021/acs.langmuir.5b03421>.

Wilson, C., Saunter, C.D., Girkin, J.M., and McCarron, J.G. (2015). Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium. J Physiol 593, 5231–5253 <doi:10.1113%2FJP271157>.

Jeppesen, J.C., Solovyeva, V., Brewer, J.R., Johannes, L., Hansen, P.L., and Simonsen, A.C. (2015). Slow Relaxation of Shape and Orientational Texture in Membrane Gel Domains. Langmuir 31, 12699–12707 <doi:10.1021%2Facs.langmuir.5b03168>.

M. Jackson, J., B. Taylor, J., A. Witek, M., A. Hunsucker, S., P. Waugh, J., Fedoriw, Y., C. Shea, T., A. Soper, S., and M. Armistead, P. (2016). Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood. Analyst. <doi:10.1039%2FC5AN01836F>.

Gualda, E.J., Pereira, H., Vale, T., Estrada, M.F., Brito, C., and Moreno, N. (2015). SPIM-fluid: open source light-sheet based platform for high-throughput imaging. Biomed. Opt. Express, BOE 6, 4447–4456 <doi:10.1364%2FBOE.6.004447>.

Schmohl, K.A., Müller, A.M., Wechselberger, A., Rühland, S., Salb, N., Schwenk, N., Heuer, H., Carlsen, J., Göke, B., Nelson, P.J., et al. (2015). Thyroid hormones and tetrac: new regulators of tumour stroma formation via integrin αvβ3. Endocr Relat Cancer 22, 941–952 <doi:10.1530%2FERC-15-0245>.

Cazaux, S., Sadoun, A., Biarnes-Pelicot, M., Martinez, M., Obeid, S., Bongrand, P., Limozin, L., and Puech, P.-H. (2016). Synchronizing atomic force microscopy force mode and fluorescence microscopy in real time for immune cell stimulation and activation studies. Ultramicroscopy 160, 168–181 <doi:10.1016%2Fj.ultramic.2015.10.014>.

Stauffer, B., and Powers, T. (2015). Target of rapamycin signaling mediates vacuolar fission caused by endoplasmic reticulum stress in S. cerevisiae. Mol. Biol. Cell mbc.E15–06 – 0344 <doi:10.1091%2Fmbc.E15-06-0344>.

Hankins, H.M., Sere, Y.Y., Diab, N.S., Menon, A.K., and Graham, T.R. (2015). Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles. Mol. Biol. Cell mbc.E15–07 – 0487 <doi:10.1091%2Fmbc.E15-07-0487>.

Yu, Y.H., Narayanan, G., Sankaran, S., Ramasamy, S., Chan, S.Y., Lin, S., Chen, J., Yang, H., Srivats, H., and Ahmed, S. (2015). Purification, Visualization, and Molecular Signature of Neural Stem Cells. Stem Cells and Development <doi:10.1089%2Fscd.2015.0190>.

Bajcsy, P., Cardone, A., Chalfoun, J., Halter, M., Juba, D., Kociolek, M., Majurski, M., Peskin, A., Simon, C., Simon, M., et al. (2015). Survey statistics of automated segmentations applied to optical imaging of mammalian cells. BMC Bioinformatics 16, 330 <doi:10.1186%2Fs12859-015-0762-2>.

Desmarais, S.M., Tropini, C., Miguel, A., Cava, F., Monds, R.D., Pedro, M.A. de, and Huang, K.C. (2015). High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography. J. Biol. Chem. jbc.M115.661660 <doi:10.1074%2Fjbc.M115.661660>.

Marquardt, L.M., Ee, X., Iyer, N., Hunter, D., Mackinnon, S.E., Wood, M.D., and Sakiyama-Elbert, S.E. (2015). Finely Tuned Temporal and Spatial Delivery of GDNF Promotes Enhanced Nerve Regeneration in a Long Nerve Defect Model. Tissue Engineering Part A 21, 2852–2864 <doi:10.1089%2Ften.tea.2015.0311> .

Lanier, M.H., Kim, T., and Cooper, J.A. (2015). CARMIL2 is a Novel Molecular Connection between Vimentin and Actin Essential for Cell Migration and Invadopodia Formation. Mol. Biol. Cell mbc.E15–08 – 0552 <doi:10.1091%2Fmbc.E15-08-0552>.

Lin, Y., Sohn, C.H., Dalal, C.K., Cai, L., and Elowitz, M.B. (2015). Combinatorial gene regulation by modulation of relative pulse timing. Nature 527, 54–58 <doi:10.1038%2Fnature15710>.

Devraj, K., Poznanovic, S., Spahn, C., Schwall, G., Harter, P.N., Mittelbronn, M., Antoniello, K., Paganetti, P., Muhs, A., Heilemann, M., et al. (2015). BACE-1 is expressed in the blood–brain barrier endothelium and is upregulated in a murine model of Alzheimer’s disease. J Cereb Blood Flow Metab 0271678X15606463 <doi:10.1177%2F0271678X15606463>.

Feutlinske, F., Browarski, M., Ku, M.-C., Trnka, P., Waiczies, S., Niendorf, T., Stallcup, W.B., Glass, R., Krause, E., and Maritzen, T. (2015). Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates focal adhesion dynamics and cell motility. Nat Commun 6, 8535 <doi:10.1038%2Fncomms9535>.

Geyer, E.A., Burns, A., Lalonde, B.A., Ye, X., Piedra, F.-A., Huffaker, T.C., and Rice, L.M. (2015). A mutation uncouples the tubulin conformational and GTPase cycles, revealing allosteric control of microtubule dynamics. eLife Sciences e10113 <doi:10.7554%2FeLife.10113>.

Bosse, J.B., Hogue, I.B., Feric, M., Thiberge, S.Y., Sodeik, B., Brangwynne, C.P., and Enquist, L.W. (2015). Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion. PNAS 112, E5725–E5733 <doi:10.1073%2Fpnas.1513876112>.

T. Brown, A., D. Vladescu, I., Dawson, A., Vissers, T., Schwarz-Linek, J., S. Lintuvuori, J., and K. Poon, W.C. (2016). Swimming in a crystal. Soft Matter 12, 131–140 <doi:10.1039%2FC5SM01831E>.

Bosse, J.B., Hogue, I.B., Feric, M., Thiberge, S.Y., Sodeik, B., Brangwynne, C.P., and Enquist, L.W. (2015). Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion. PNAS 112, E5725–E5733 <doi:10.1073%2Fpnas.1513876112>.

Liu, X., Qu, X., Jiang, Y., Chang, M., Zhang, R., Wu, Y., Fu, Y., and Huang, S. (2015). Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth. Molecular Plant 8, 1694–1709 <doi:10.1016%2Fj.molp.2015.09.013>.

Puah, W.C., and Wasser, M. Live imaging of muscles in Drosophila metamorphosis: Towards high-throughput gene identification and function analysis. Methods <doi:10.1016/j.ymeth.2015.09.028>.

Hadad, U., Thauland, T.J., Martinez, O.M., Butte, M.J., Porgador, A., and Krams, S.M. (2015). NKp46 clusters at the immune synapse and regulates NK cell polarization. Front. Immunol. 495 <doi:10.3389%2Ffimmu.2015.00495>.

Berenguer, J.R., Pichel, J.G., Giménez, N., Lalinde, E., Moreno, M.T., and Piñeiro-Hermida, S. (2015). Luminescent pentafluorophenyl-cycloplatinated complexes: synthesis, characterization, photophysics, cytotoxicity and cellular imaging. Dalton Trans. 44, 18839–18855 <doi:10.1039%2FC5DT02721G>.

Jonas, R., Klusch, A., Schmelz, M., Petersen, M., and Carr, R.W. (2015). Assessment of TTX-s and TTX-r Action Potential Conduction along Neurites of NGF and GDNF Cultured Porcine DRG Somata. PLoS ONE 10, e0139107 <doi:10.1371%2Fjournal.pone.0139107>.

Zou, W., Yadav, S., DeVault, L., Jan, Y.N., and Sherwood, D.R. (2015). RAB-10-Dependent Membrane Transport Is Required for Dendrite Arborization. PLoS Genet 11, e1005484 <doi:10.1371%2Fjournal.pgen.1005484>.

Gimber, N., Tadeus, G., Maritzen, T., Schmoranzer, J., and Haucke, V. (2015). Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins. Nat Commun 6, 8392 <doi:10.1038%2Fncomms9392>.

Snijders, T., Smeets, J.S.J., van Kranenburg, J., Kies, A.K., van Loon, L.J.C., and Verdijk, L.B. (2015). Changes in myonuclear domain size do not precede muscle hypertrophy during prolonged resistance-type exercise training. Acta Physiol n/a – n/a <doi:10.1111%2Fapha.12609>.

Walck-Shannon, E., Reiner, D., and Hardin, J. (2015). Polarized Rac-dependent protrusions drive epithelial intercalation in the embryonic epidermis of C. elegans. Development dev.127597 <doi:10.1242%2Fdev.127597>.

Lukacs, V., Mathur, J., Mao, R., Bayrak-Toydemir, P., Procter, M., Cahalan, S.M., Kim, H.J., Bandell, M., Longo, N., Day, R.W., et al. (2015). Impaired PIEZO1 function in patients with a novel autosomal recessive congenital lymphatic dysplasia. Nat Commun 6, 8329 <doi:10.1038%2Fncomms9329>.

Mary, H., Fouchard, J., Gay, G., Reyes, C., Gauthier, T., Gruget, C., Pécréaux, J., Tournier, S., and Gachet, Y. (2015). Fission yeast kinesin-8 controls chromosome congression independently of oscillations. J Cell Sci 128, 3720–3730 <doi:10.1242%2Fjcs.160465>.

Martínez, L.E., Hardcastle, J.M., Wang, J., Pincus, Z., Tsang, J., Hoover, T.R., Bansil, R., and Salama, N.R. (2016). Helicobacter pylori strains vary cell shape and flagellum number to maintain robust motility in viscous environments. Molecular Microbiology 99, 88–110 <doi:10.1111%2Fmmi.13218>.

Paul, N.R., Allen, J.L., Chapman, A., Morlan-Mairal, M., Zindy, E., Jacquemet, G., Ama, L.F. del, Ferizovic, N., Green, D.M., Howe, J.D., et al. (2015). α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3. J Cell Biol 210, 1013–1031 <doi:10.1083%2Fjcb.201502040>.

Bhattacharjee, S. (2015). Emerging trend of advanced image analysis in nanomedicine. Nanomedicine 10, 2801–2804 <doi:10.2217%2Fnnm.15.121>.

Wendykier, P., Borucki, B., and Nowinski, K.S. (2015). Large Java arrays and their applications. In 2015 International Conference on High Performance Computing Simulation (HPCS), pp. 460–467 <doi:10.1109%2FHPCSim.2015.7237077>.

Lloyd, D.P., and Allen, R.J. (2015). Competition for space during bacterial colonization of a surface. Journal of The Royal Society Interface 12, 20150608 <doi:10.1098%2Frsif.2015.0608>.

Windschüttl, S., Nettersheim, D., Schlatt, S., Huber, A., Welter, H., Schwarzer, J.U., Köhn, F.M., Schorle, H., and Mayerhofer, A. (2014). Are testicular mast cells involved in the regulation of germ cells in man? Andrology 2, 615–622 <doi:10.1111%2Fj.2047-2927.2014.00227.x>.

Ingaramo, M., York, A.G., Andrade, E.J., Rainey, K., and Patterson, G.H. (2015). Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence. Nat Commun 6, 8184 <doi:10.1038%2Fncomms9184>.

Watanabe, T., Kakeno, M., Matsui, T., Sugiyama, I., Arimura, N., Matsuzawa, K., Shirahige, A., Ishidate, F., Nishioka, T., Taya, S., et al. (2015). TTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation. J Cell Biol 210, 737–751 <doi:10.1083%2Fjcb.201412075>.

Uchida, A., Monsma, P.C., Fenn, J.D., and Brown, A. Live-cell imaging of neurofilament transport in cultured neurons. B.-M. in C. Biology, ed. (Academic Press), link.

Grant, M.P., Cavanaugh, A., and Breitwieser, G.E. (2015). 14-3-3 Proteins Buffer Intracellular Calcium Sensing Receptors to Constrain Signaling. PLoS ONE 10, e0136702 <doi:10.1371%2Fjournal.pone.0136702>.

Nirschl, J.J., and Holzbaur, E.L.F. Live-cell imaging of retrograde transport initiation in primary neurons. B.-M. in C. Biology, ed. (Academic Press), link.

Pajović, J.D., Dojčilović, R., Božanić, D.K., Kaščáková, S., Réfrégiers, M., Dimitrijević-Branković, S., Vodnik, V.V., Milosavljević, A.R., Piscopiello, E., Luyt, A.S., et al. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. Colloids and Surfaces B: Biointerfaces 135, 742–750 <doi:10.1016%2Fj.colsurfb.2015.08.050>.

Wabnig, S., Liewald, J.F., Yu, S., and Gottschalk, A. (2015). High-Throughput All-Optical Analysis of Synaptic Transmission and Synaptic Vesicle Recycling in Caenorhabditis elegans. PLoS ONE 10, e0135584 [](http://dx.doi.org/10.1371/journal.pone.0135584).

Chazeau, A., Katrukha, E.A., Hoogenraad, C.C., and Kapitein, L.C. Studying neuronal microtubule organization and microtubule-associated proteins using single molecule localization microscopy. B.-M. in C. Biology, ed. (Academic Press),link.

Khayatan, B., Meeks, J.C., and Risser, D.D. (2015). Evidence that a modified type IV pilus-like system powers gliding motility and polysaccharide secretion in filamentous cyanobacteria. Molecular Microbiology 98, 1021–1036 <doi:10.1111%2Fmmi.13205>.

Ellefsen, K.L., Dynes, J.L., and Parker, I. (2015). Spinning-Spot Shadowless TIRF Microscopy. PLoS ONE 10, e0136055 <doi:10.1371%2Fjournal.pone.0136055>.

Dietrich, M.G., Zuellig, R.A., Spinas, G.A., Lehmann, R., Tschopp, O., and Niessen, M. (2015). Specific and redundant roles of PKBα/AKT1 and PKBβ/AKT2 in human pancreatic islets. Experimental Cell Research 338, 82–88 <doi:10.1016%2Fj.yexcr.2015.08.008>.

Adames, N.R., Schuck, P.L., Chen, K.C., Murali, T.M., Tyson, J.J., and Peccoud, J. (2015). Experimental testing of a new integrated model of the budding yeast START transition. Mol. Biol. Cell [doi:mbc.E15–06](doi:mbc.E15–06) – 0358.

Reubold, T.F., K. Faelber, N. Plattner, Y. Posor, K. Ketel, U. Curth, J. Schlegel, R. Anand, D.J. Manstein, F. Noé, V. Haucke, O. Daumke, and S. Eschenburg. 2015. Crystal structure of the dynamin tetramer. Nature. <doi:10.1038/nature14880>.

Tanenbaum, M.E., N. Stern-Ginossar, J.S. Weissman, and R.D. Vale. 2015. Regulation of mRNA translation during mitosis. eLife. 4. <doi:10.7554/eLife.07957>.

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Kim, J.H., Y. Ren, W.P. Ng, S. Li, S. Son, Y.-S. Kee, S. Zhang, G. Zhang, D.A. Fletcher, D.N. Robinson, and E.H. Chen. Mechanical Tension Drives Cell Membrane Fusion. Developmental Cell. <doi:10.1016/j.devcel.2015.01.005>.

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2014

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1. Electrochemical Regulation of Budding Yeast Polarity. PLoS Biol. 12:e1002029. <doi:10.1371/journal.pbio.1002029>.

Shultzaberger, R.K., M.L. Paddock, T. Katsuki, R.J. Greenspan, and S.S. Golden. 2015. Chapter Three - High-Throughput and Quantitative Approaches for Measuring Circadian Rhythms in Cyanobacteria Using Bioluminescence. In Methods in Enzymology. A. Sehgal, editor. Academic Press. 53–72.

DeWitt, B.N., and R.C. Dunn. 2014. Interaction of Cholesterol in Ternary Lipid Mixtures Investigated Using Single-Molecule Fluorescence. Langmuir. 31:995–1004. <doi:10.1021/la503797w>.

Selimkhanov, J., B. Taylor, J. Yao, A. Pilko, J. Albeck, A. Hoffmann, L. Tsimring, and R. Wollman. 2014. Accurate information transmission through dynamic biochemical signaling networks. Science. 346:1370–1373. <doi:10.1126/science.1254933>.

Fincher, Jarod, Canaan Whiteneck, and Eric Birgbauer. “G-protein-coupled receptor cell signaling pathways mediating embryonic chick retinal growth cone collapse induced by lysophosphatidic Acid and sphingosine-1-phosphate.” Developmental neuroscience 36.6 (2013): 443-453.

Wang, J., J.W. Petefish, A.C. Hillier, and I.C. Schneider. 2014. Epitaxially Grown Collagen Fibrils Reveal Diversity in Contact Guidance Behavior among Cancer Cells. Langmuir. <doi:10.1021/la503254x>.

Tao-Tao Liu, Zhi-Gang He, Xue-Bi Tian, Hong-Bing Xiang: Neural mechanisms and potential treatment of epilepsy and its complications. Am J Transl Res 2014;6(6):625-630. (Abstract AJTR0002907, Full text, PDF).

Yen-Ta Chen, Chih-Chao Yang, Cheuk-Kwan Sun, Hsin-Ju Chiang, Yi-Ling Chen, Pei-Hsun Sung, Yen-Yi Zhen, Tein-Hung Huang, Chia-Lo Chang, Hong-Hwa Chen, Hsueh-Wen Chang, Hon-Kan Yip: Extracorporeal shock wave therapy ameliorates cyclophosphamide-induced rat acute interstitial cystitis though inhibiting inflammation and oxidative stress-in vitro and in vivo experiment studies. Am J Transl Res 2014;6(6):631-648. (Abstract AJTR0002520, Full text, PDF).

Longfei Huo, Chia-Wei Li, Tzu-Hsuan Huang, Yung Carmen Lam, Weiya Xia, Chun Tu, Wei-Chao Chang, Jennifer L Hsu, Dung-Fang Lee, Lei Nie, Hirohito Yamaguchi, Yan Wang, Jingyu Lang, Long-Yuan Li, Chung-Hsuan Chen, Lopa Mishra, Mien-Chie Hung: Activation of Keap1/Nrf2 signaling pathway by nuclear epidermal growth factor receptor in cancer cells. Am J Transl Res 2014;6(6):649-663. (Abstract AJTR0002147, Full text, PDF).

Xiaojie Wei, Songbin He, Zhouguang Wang, Jiamin Wu, Jinjing Zhang, Yi Cheng, Jie Yang, Xinlong Xu, Zaifeng Chen, Junmin Ye, Li Chen, Li Lin, Jian Xiao: Fibroblast growth factor 1 attenuates 6-hydroxydopamine-induced neurotoxicity: an in vitro and in vivo investigation in experimental models of parkinson’s disease. Am J Transl Res 2014;6(6):664-677. (Abstract AJTR0001987, Full text, PDF).

Weijuan Li, Yaoqi Chen, Songhai Li, Xiaopeng Guo, Wenping Zhou, Qiutang Zeng, Yuhua Liao, Yumiao Wei: Agonistic antibody to angiotensin II type 1 receptor accelerates atherosclerosis in ApoE-/- mice. Am J Transl Res 2014;6(6):678-690. (Abstract AJTR0002772, Full text, PDF).

Tingting Chen, Pei Zhang, Wenxia Fan, Fenghua Qian, Li Pei, Shuangnian Xu, Zhongmin Zou, Bing Ni, Yong Zhang: Co-transplantation with mesenchymal stem cells expressing a SDF-1/HOXB4 fusion protein markedly improves hematopoietic stem cell engraftment and hematogenesis in irradiated mice. Am J Transl Res 2014;6(6):691-702.

Jing-Juan Huang,Yi-Qin Shi, Rui-Lin Li, An Hu, Hong-Sheng Zhou, Qian Cheng, Zheng Xu, Zhi-Ming Yang, Chang-Ning Hao, Jun-Li Duan: Angiogenesis effect of therapeutic ultrasound on ischemic hind limb in mice. Am J Transl Res 2014;6(6):703-713. (Abstract AJTR0002564, Full text, PDF).

Hai Fan, Likun Ma, Bin Fan, Jiawei Wu, Zhe Yang, Lei Wang: Role of PDGFR-β/PI3K/AKT signaling pathway in PDGF-BB induced myocardial fibrosis in rats. Am J Transl Res 2014;6(6):714-723. (Abstract AJTR0001969, Full text, PDF).

Paul W Burridge, Scott A Metzler, Karina H Nakayama, Oscar J Abilez, Chelsey S Simmons, Marc A Bruce, Yuka Matsuura, Paul Kim, Joseph C Wu, Manish Butte, Ngan F Huang, Phillip C Yang: Multi-cellular interactions sustain long-term contractility of human pluripotent stem cell-derived cardiomyocytes. Am J Transl Res 2014;6(6):724-735. (Abstract AJTR0002357, Full text, PDF).

Ledderose, C., Y. Bao, J. Zhang, and W.G. Junger. 2014. Novel method for real-time monitoring of ATP release reveals multiple phases of autocrine purinergic signaling during immune cell activation. Acta Physiol. n/a–n/a. <doi:10.1111/apha.12435>.

Dufour, A., T. Liu, C. Ducroz, R. Tournemenne, B. Cummings, R. Thibeaux, N. Guillen, A. Hero, and J. Olivo-Marin. 2015. Signal Processing Challenges in Quantitative 3-D Cell Morphology: More than meets the eye. IEEE Signal Processing Magazine. 32:30–40. <doi:10.1109/MSP.2014.2359131>.

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Tuson, H. H., Auer, G. K., Renner, L. D., Hasebe, M., Tropini, C., Salick, M., Crone, W. C., Gopinathan, A., Huang, K. C. and Weibel, D. B. (2012). Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity. Molecular Microbiology DOI: 10.1111/j.1365-2958.2012.08063.x

Jonas Ries, Charlotte Kaplan, Evgenia Platonova, Hadi Eghlidi & Helge Ewers (2012). A simple, versatile method for GFP-based super-resolution microscopy via nanobodies. Nature Methods DOI: 10.1038/nmeth.1991

Subramanian Krishnan, G. Esteban Fernandez, David B. Sacks, Nemani V. Prasadarao (2012). IQGAP1 mediates the disruption of adherens junctions to promote Escherichia coli K1 invasion of brain endothelial cells. Cellular Microbiology DOI: 10.1111/j.1462-5822.2012.01805.x

R F Hennigan, C A Moon, L M Parysek, K R Monk, G Morfini, S Berth, S Brady and N Ratner (2012). The NF2 tumor suppressor regulates microtubule-based vesicle trafficking via a novel Rac, MLK and p38SAPK pathway. Oncogene DOI: 10.1038/onc.2012.135

Dirk Landgraf, Burak Okumus, Peter Chien, Tania A Baker and Johan Paulsson (2012). Segregation of molecules at cell division reveals native protein localization. Nature Methods <DOI:10.1038/nmeth.1955>

Moritz Lang, Fabian Rudolf, Jörg Stelling (2012). Use of YouScope to Implement Systematic Microscopy Protocols. Current Protocols in Molecular Biology DOI: 10.1002/0471142727.mb1421s98

Erich E. Hoover1, Jeffrey J. Field, David G. Winters, Michael D. Young, Eric V. Chandler, John C. Speirs, Jacob T. Lapenna, Susy M. Kim, Shi-you Ding, Randy A. Bartels, Jing W. Wang, and Jeff A. Squier1 (2012). Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems. Journal of Biophotonics DOI: 10.1002/jbio.201100139

Travis J. Crites, Lirong Chen, and Rajat Varma (2012). A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins. Journal of Visualized Experiments DOI: 10.3791/3892

Wei Li, Wei Zou, Yihong Yang, Yongping Chai, Baohui Chen, Shiya Cheng, Dong Tian, Xiaochen Wang, Ronald D. Vale, and Guangshuo Ou (2012). Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell. Journal of Cell Biology DOI: 10.1083/jcb.201111053

Z. Schilling, E. Frank, V. Magidson, J. Wason, J. Loncarek, K. Boyer, J. Wen, and A. Khodjakov (2012). Predictive-focus illumination for reducing photodamage in live-cell microscopy. Journal of Microscopy DOI: 10.1111/j.1365-2818.2012.03605.x

Saumya Saurabh, Suvrajit Maji, and Marcel P. Bruchez (2012). Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules. Optics Express DOI: 10.1364/OE.20.007338

Junyu Mai, Greg J. Sommer, and Anson V. Hatch (2012). Microfluidic Digital Isoelectric Fractionation for Rapid Multi-Dimensional Glycoprotein Analysis. Analytical Chemistry DOI: 10.1021/ac203076p

Petar Marinković, Miriam S. Reuter, Monika S. Brill, Leanne Godinho, Martin Kerschensteiner, and Thomas Misgeld (2012). Axonal transport deficits and degeneration can evolve independently in mouse models of amyotrophic lateral sclerosis. PNAS DOI: 10.1073/pnas.1200658109

Stefanie Kaech, Chun-Fang Huang, and Gary Banker (2012). General Considerations for Live Imaging of Developing Hippocampal Neurons in Culture. Cold Spring Harb Protocols DOI: 10.1101/pdb.ip068221

Andrew J. Ewald, Robert J. Huebner, Hildur Palsdottir, Jessie K. Lee, Melissa J. Perez, Danielle M. Jorgens, Andrew N. Tauscher, Kevin J. Cheung, Zena Werb, and Manfred Auer (2012). Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium. Journal of Cell Sci jcs.096875 DOI: 10.1242/jcs.096875

Vedakumar Tatavarty, Marius F. Ifrim, Mikhail Levin, George Korza, Elisa Barbarese, Ji Yu, and John H. Carson (2012). Single molecule imaging of translational output from individual RNA granules in neurons. Mol. Biol. Cell mbc.E11-07-0622 DOI: 10.1091/mbc.E11-07-0622

Joshua M. Katzenstein, Dustin W. Janes, Haley E. Hocker, Justin K. Chandler, and Christopher J. Ellison (2012). Nanoconfined Self-Diffusion of Poly(isobutyl methacrylate) in Films with a Thickness-Independent Glass Transition. Macromolecules 45 (3), 1544-1552 DOI: 10.1021/ma202362j

Deanna M. Navaroli, Karl D. Bellvé, Clive Standley, Lawrence M. Lifshitz, James Cardia, David Lambright, Deborah Leonard, Kevin E. Fogarty, and Silvia Corvera (2012). Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. PNAS <DOI:10.1073/pnas.1115495109>

Remi Renaud, Claire Martin, Hirac Gurden, and Frederic Pain (2012). Multispectral reflectance imaging of brain activation in rodents: methodological study of the differential path length estimations and first in vivo recordings in the rat olfactory bulb. Journal of Biomedical Optics 17, 016012 DOI: 10.1117/1.JBO.17.1.016012

André Lampe, Volker Haucke, Stephan J. Sigrist, Mike Heilemann, Jan Schmoranzer (2012). Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell DOI: 10.1111/boc.201100011

J. Bradley Zuchero, Brittany Belin, and R. Dyche Mullins (2012). Actin Binding to WH2 Domains Regulates Nuclear Import of the Multifunctional Actin Regulator JMY. Molecular Biology of The Cell DOI: mbc.E11-12-0992

Jean-Cheng Kuo, Xuemei Han, John R. Yates and Clare M. Waterman (2012). Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis. Methods in Molecular Biology: Integrin and Cell Adhesion Molecules, Volume 757, Part 4, 297-323 DOI: 10.1007/978-1-61779-166-6_19

Vladimir Lizunov, Karin Stenkula, Ivonne Lisinski, Oksana Gavrilova, Dena R. Yver, Alexandra Chadt, H Al-Hasani, Joshua Zimmerberg, and Samuel W. Cushman (2012). Insulin stimulates fusion, but not tethering, of GLUT4 vesicles in skeletal muscle of transgenic mouse. American Journal of Physiology DOI: 10.1152/ajpendo.00466.2011

Subhasri Ghosh, Suvrajit Saha, Debanjan Goswami, Sameera Bilgrami, Satyajit Mayor, Chapter sixteen - Dynamic Imaging of Homo-FRET in Live Cells by Fluorescence Anisotropy Microscopy, In: P.Michael Conn, Editor(s), Methods in Enzymology, Academic Press, 2012, Volume 505, Pages 291-327, ISSN 0076-6879. DOI: 10.1016/B978-0-12-388448-0.00024-3

Total 2012 citation: 96

2011

Christian Conrad, Annelie Wünsche, Tze Heng Tan, Jutta Bulkescher, Frank Sieckmann, Fatima Verissimo, Arthur Edelstein, Thomas Walter, Urban Liebel, Rainer Pepperkok & Jan Ellenberg (2011). Micropilot: automation of fluorescence microscopy-based imaging for systems biology. Nature Methods 1548-7091 DOI: 10.1038/nmeth.1558

Dimiter Prodanov (2011). Data Ontology and an Information System Realization for Web-Based Management of Image Measurements. Frontiers in Neuroinformatics DOI: 10.3389/fninf.2011.00025

Iris Sarchizian, Marinela Cirnu, Ioan I. Ardelean (2011). Isolation of a heterocyts - forming Cyanobacterium and quantification of its biotechnological potential with respect to redox properties at single cell level. Romanian Biotechnological Letters Vol. 16, No.6, 2011, Supplement DOI: PDF file

Shawna C. O. Reed, Alisa W. Serio, Matthew D. Welch (2011), Rickettsia parkeri invasion of diverse host cells involves an Arp2/3 complex, WAVE complex and Rho-family GTPase-dependent pathway. Cellular Microbiology 1462-5822. DOI: 10.1111/j.1462-5822.2011.01739.x

Rose Loughlin, Jeremy D. Wilbur, Francis J. McNally, François J. Nédélec, Rebecca Heald (2011), Katanin Contributes to Interspecies Spindle Length Scaling in Xenopus. Cell Volume 147, Issue 6. DOI: 10.1016/j.cell.2011.11.014

Hench J , Bratić Hench I , Pujol C , Ipsen S , Brodesser S , et al. (2011) A Tissue-Specific Approach to the Analysis of Metabolic Changes in Caenorhabditis elegans. PLoS ONE 6(12): e28417. DOI: 10.1371/journal.pone.0028417

Milanova, D., Chambers, R. D., Bahga, S. S. and Santiago, J. G. (2011), Electrophoretic mobility measurements of fluorescent dyes using on-chip capillary electrophoresis. ELECTROPHORESIS, 32: 3286–3294. DOI: 10.1002/elps.201100210

Taizo Kawano1, Michelle D. Po1, Shangbang Gao1, George Leung, William S. Ryu, Mei Zhen (2011), An Imbalancing Act: Gap Junctions Reduce the Backward Motor Circuit Activity to Bias C. elegans for Forward Locomotion. Neuron Volume 72, Issue 4 DOI: 10.1016/j.neuron.2011.09.005

Yuli Wang, Pavak Shah, Colleen Phillips, Christopher E. Sims and Nancy L. Allbritton (2011), Trapping cells on a stretchable microwell array for single-cell analysis. Analytical and Bioanalytical Chemistry DOI: 10.1007/s00216-011-5535-9

Danny A. Stark, Rowan M. Karvas, Ashley L. Siegel, and D. D. W. Cornelison (2011), Eph/ephrin interactions modulate muscle satellite cell motility and patterning. Development DOI: 10.1242/dev.068411

Leavesley, S. J., Annamdevula, N., Boni, J., Stocker, S., Grant, K., Troyanovsky, B., Rich, T. C. and Alvarez, D. F. (2011), Hyperspectral imaging microscopy for identification and quantitative analysis of fluorescently-labeled cells in highly autofluorescent tissue. Journal of Biophotonics DOI: 10.1002/jbio.201100066

Patent: Chambers, Robert D. (Enfield, NH, US), Santiago, Juan G. (Stanford, CA, US), Bercovici, Moran (Santa Clara, CA, US) 2011. Non-focusing tracers for indirect detection in electrophoretic displacement techniques. United States 20110220499

Yinglang Wan, William M Ash, Lusheng Fan, Huaiqin Hao, Myung K Kim and Jinxing Lin (2011). Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana. Plant Methods 7:27. <DOI:10.1186/1746-4811-7-27>

Jared E Toettcher, Delquin Gong, Wendell A Lim & Orion D Weiner (2011). Light-based feedback for controlling intracellular signaling dynamics. Nature Methods 8, 1548-7105. <DOI:10.1038/nmeth.1700>

Guillermo A. Yudowski and Mark von Zastrow (2011). Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM. Methods in Molecular Biology 756:325-332. DOI: 10.1007/978-1-61779-160-4_19

Thai V Truong, Willy Supatto, David S Koos, John M Choi & Scott E Fraser (2011). Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods 8, 757–760 (2011) <doi:10.1038/nmeth.1652>

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}]

Koen J. C. Verbrugghe, Raymond C. Chan (2011). Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software. Journal of Visualized Experiments <DOI:10.3791/2625>

Maarten H.P. Kole (2011). First Node of Ranvier Facilitates High-Frequency Burst Encoding. Neuron 71:671-682. doi: 10.1016/j.neuron.2011.06.024

Sanchari Bhattacharya, Tzu-Chiao Chao, and Alexandra Ros (2011). Insulator-based dielectrophoretic single particle and single cancer cell trapping. Electrophoresis DOI: 10.1002/elps.201100066

Stanislav Vitha and Katherine W. Osteryoung (2011). Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins. Methods in Molecular Biology 774:33-58 DOI: 10.1007/978-1-61779-234-2_3

Benoît Kornmann, Christof Osman, and Peter Walter (2011). The conserved GTPase Gem1 regulates endoplasmic reticulum–mitochondria connections. PNAS doi: 10.1073/pnas.1111314108

Young-Hwan Song, Euna Choi, Sun-Hyun Park, Suk-Ho Lee, Hana Cho, Won-Kyung Ho, and Shin-Young Ryu (2011). Sustained CaMKII activity mediates transient oxidative stress-induced long-term facilitation of L-type Ca2+ current in cardiomyocytes. Free Radical Biology and Medicine <doi:10.1016/j.freeradbiomed.2011.07.022>

Asuka Nakano, Tzu-Chiao Chao, Fernanda Camacho-Alanis, and Alexandra Ros (20111). Immunoglobulin G and bovine serum albumin streaming dielectrophoresis in a microfluidic device. Electrophoresis. In Press. doi: 10.1002/elps.201100037

Y Wakamoto, A.Y. Grosberg, and E. Kussell (2011). Optimal lineage principle for age-structured populations. Evolution In Press. doi: 10.1111/j.1558-5646.2011.01418.x

Eric Campeau (2011). RNA interference in mammals: behind the screen. Briefings in Functional Genomics 10:215-226. doi: 10.1093/bfgp/elr018

Ivo A. Telley, Peter Bieling and Thomas Surrey (2011). Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy. Methods in Molecular Biology 777:127-145 doi: 10.1007/978-1-61779-252-6_10

Aaron G. Blankenship, Aaron M. Hamby, Alana Firl, Shri Vyas, Stephan Maxeiner, Klaus Willecke, and Marla B. Feller (2011). The Role of Neuronal Connexins 36 and 45 in Shaping Spontaneous Firing Patterns in the Developing Retina. The Journal of Neuroscience 31:9998-10008 doi: 10.1523/​JNEUROSCI.5640-10.2011

Min S. Wang, Joshua C. Black, Michelle K. Knowles and Scott M. Reed (2011). C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP. Analytical and BioAnalytical Chemistry doi: 10.1007/s00216-011-5174-1

Keren K. Griffiths, Jingqiao Zhang, Ann E. Cowan, Ji Yu, Peter Setlow (2011). Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster. Molecular Microbiology DOI: 10.1111/j.1365-2958.2011.07753.x

Poole RJ, Bashllari E, Cochella L, Flowers EB, Hobert O, (2011) A Genome-Wide RNAi Screen for Factors Involved in Neuronal Specification in Caenorhabditis elegans. PLoS Genet 7(6): e1002109. <doi:10.1371/journal.pgen.1002109>

Godefroid Charbon, Eric Brustad, Kevin A. Scott, Jiangyun Wang, Anders Løbner-Olesen, Peter G. Schultz, Christine Jacobs-Wagner, and Eli Chapman (2011). Subcellular Protein Localization by Using a Genetically Encoded Fluorescent Amino Acid. ChemBioChem 12: n/a.DOI: 10.1002/cbic.201100282

Luciano Marpegan, Adrienne E. Swanstrom, Kevin Chung, Tatiana Simon, Philip G. Haydon, Sanjoy K. Khan, Andrew C. Liu, Erik D. Herzog, and Christian Beaulé (2011). Circadian Regulation of ATP Release in Astrocytes. The Journal of Neuroscience, 31: 8342-8350. doi: 10.1523/​JNEUROSCI.6537-10.2011

Michael Carnell, Tobias Zech, Simon D. Calaminus, Seiji Ura, Monica Hagedorn, Simon A. Johnston, Robin C. May, Thierry Soldati, Laura M. Machesky, and Robert H. Insall (2011). Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. JCB doi: 10.1083/jcb.201009119

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}]

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 21558154 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Jing Li, Nima Najand, Wendy Long and Andrew Simmonds (2011). Imaging the Cellular Dynamics of Drosophila Argonaute Proteins. Methods in Molecular Biology. 725, 143-159 <doi:0.1007/978-1-61779-046-1_10>. PMID 21528452.

Looney, M.R., Thornton, E.E., Sen, D., Lamm, W.J., Glenny, R.W., and Krummel, M.F. (2011). Stabilized imaging of immune surveillance in the mouse lung. Nat Methods. 8, 91–96 <doi:10.1038/nmeth.1543> PMID 21151136 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Debjani Paul, Laure Saias, Jean-Cedric Pedinotti, Max Chabert, Sebastien Magnifico, Antoine Pallandre, Bertrand De Lambert, Claude Houdayer, Bernard Brugg, Jean-Michel Peyrin, and Jean-Louis Viovy (2011). A “dry and wet hybrid” lithography technique for multilevel replication templates: Applications to microfluidic neuron culture and two-phase global mixing. BioMicrofluidics 5, 024102 <doi:10.1063/1.3569946>

Heather I. C. Dalgarno, Paul A. Dalgarno, Adetunmise C. Dada, Catherine E. Towers, Gavin J. Gibson, Richard M. Parton, Ilan Davis, Richard J. Warburton and Alan H. Greenaway (2011). Nanometric depth resolution from multi-focal images in microscopy. Interface doi: 10.1098/​rsif.2010.0508 PMID 21247948 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Estrella Guarino, Marianne E. A. Shepherd, Israel Salguero, Hui Hua, Rachel S. Deegan and Stephen E. Kearsey (2011). Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation. Nucleic Acids Research doi: 10.1093/nar/gkr222 PMID 21493688

Andrea E. Knowlton, Heather M. Brown, Theresa S. Richards, Lauren A. Andreolas, Rahul K. Patel, Scott S. Grieshaber. (2011). Chlamydia trachomatis infection causes mitotic spindle pole defects independently from its effects on centrosome amplification. Traffic doi: 10.1111/j.1600-0854.2011.01204.x PMID 21477082 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Ulrich Y. Schaff1 and Greg J. Sommer (2011). Whole Blood Immunoassay Based on Centrifugal Bead Sedimentatio. Clinical Chemistry <doi:10.1373/clinchem.2011.162206> PMID 21415382

Rosario Scalia, Yulan Gong, Brett Berzins, Brin Freund, Danielle Feather, and Gavin Landesberg Gourav Mishra (2011). A Novel Role for Calpain in the Endothelial Dysfunction Induced by Activation of Angiotensin II Type 1 Receptor Signaling. Circulation Research <doi:10.1161/CIRCRESAHA.110.229393> PMID 21415394

Claudia Rubio, David Pincus, Alexei Korennykh, Sebastian Schuck, Hana El-Samad, and Peter Walter (2011). Homeostatic adaptation to endoplasmic reticulum stress depends on Ire1 kinase activity. J Cell Biol 2011 <doi:10.1083/jcb.201007077> PMID 21444688 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Charles A. Harris, Joel T. Haas, Ryan S. Streeper, Scot J. Stone, Manju Kumari, Kui Yang, Xianlin Han, Nicholas Brownell, Richard W. Gross, Rudolf Zechner and Robert V. Farese Jr (2011). DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes. The Journal of Lipid Research, 52 657 <doi:10.1194/jlr.M013003> PMID 21317108

PMID 21545698

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 21296409 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Devrim Kilinc, Jean-Michel Peyrin, Vanessa Soubeyre, Sebastien Magnifico, Laure Saias, Jean-Louis Viovy, and Bernard Brug. (2011). Wallerian-Like Degeneration of Central Neurons After Synchronized and Geometrically Registered Mass Axotomy in a Three-Compartmental Microfluidic Chip. Neurotox Res. 19:149-161. <doi:10.1007/s12640-010-9152-8> PMID 20162389 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Christopher M. Brown, Manisha Ray, Aura A. Eroy-Reveles, Pascal Egea, Cheryl Tajon, and Charles S. Craik. (2011). Peptide Length and Leaving-Group Sterics Influence Potency of Peptide Phosphonate Protease Inhibitors. Chemistry & Biology 18:48-57 <doi:10.1016/j.chembiol.2010.11.007> PMID 21276938 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Matthew I. Hoopes, Roland Faller, and Marjorie L. Longo. (2011). Lipid Domain Depletion at Small Localized Bends Imposed by a Step Geometry. Langmuir 27:2783-2788 <doi:10.1021/la104504p> PMID 21338070

Ricardo Henriques, Caron Griffiths, E. Hesper Rego, and Musa M. Mhlanga (2011). PALM and STORM: Unlocking live-cell super-resolution. Biopolymers 95:322-331 <doi:10.1002/bip.21586> PMID 21254001

Rachel McMullan, and Stephen J. Nurrish. (2011). The RHO-1 RhoGTPase Modulates Fertility and Multiple Behaviors in Adult C. elegans. PLoS ONE (2): e17265. <doi:10.1371/journal.pone.0017265> PMID 21387015 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Saravanan Rajan, Haig Djambazian, Huan Chu Pham Dang, Rob Sladek, and Thomas J Hudson. (2011). The living microarray: a high-throughput platform for measuring transcription dynamics in single cells. BMC Genomics 12:115. <doi:10.1186/1471-2164-12-115> PMID 21324195 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Hui Hua, and Stephen E. Kearsey (2011). Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2′-deoxyuridine. Nucl. Acids Res. <doi:10.1093/nar/gkr063> PMID 21310713 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Nicolas Minc, David Burgess, and Fred Chang. (2011). Influence of Cell Geometry on Division-Plane Positioning. Cell 144: 427-438 <doi:10.1016/j.cell.2011.01.016> PMID 21295701 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Christian Conrad, Annelie Wünsche, Tze Heng Tan, Jutta Bulkescher,Frank Sieckmann, Fatima Verissimo, Arthur Edelstein, Thomas Walter, Urban Liebel, Rainer Pepperkok & Jan Ellenberg. (2011). Micropilot: automation of fluorescence microscopy–based imaging for systems biology. Nature Methods 8:246–249 <doi:10.1038/nmeth.1558> PMID 21258339 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Total 2011 citations: 57

2010

Bruno Afonso, Pamela A. Silver, and Caroline M. Ajo-Franklin (2011). A synthetic circuit for selectively arresting daughter cells to create aging populations. Nucleic Acids Res. 38:2727–2735. doi: 10.1093/nar/gkq075

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 19926785 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 20221784 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}]

Supreet S. Bahga, Moran Bercovici, Juan G. Santiago (2011). Ionic strength effects on electrophoretic focusing and separations. Electrophoresis 31:910-919 DOI: 10.1002/elps.200900560 PMID 20191554

Thorsten Wohland, Xianke Shi, Jagadish Sankaran, and Ernst H.K. Stelzer (2010). Single Plane Illumination Fluorescence Correlation Spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments. Optics Express 18: 10627-10641 <doi:10.1364/OE.18.010627> PMID 20588915

David F. Savage, Bruno Afonso, Anna H. Chen and Pamela A. Silver (2010). Spatially Ordered Dynamics of the Bacterial Carbon Fixation Machinery Science 327:1258-1261 DOI: 10.1126/science.1186090 PMID 20203050

Fanjie Meng, Thams M. Suchyna, Elena Lazakovitch, Richard M. Gronostajski, and Frederick Sachs (2010). Real Time FRET Based Detection of Mechanical Stress in Cytoskeletal and Extracellular Matrix Proteins. Cellular and Molecular Bioengineering published online Sept. 2010 DOI: 10.1007/s12195-010-0140-0

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 20625545 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 20798350 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] PMID 21111236 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

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Liu, A.P., Aguet, F., Danuser, G., and Schmid, S.L. (2010). Local clustering of transferrin receptors promotes clathrin-coated pit initiation. JCB 191:1381-1393. doi: 10.1083/jcb.201008117 PMID 21187331 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Razooky, B.S., and Weinberger, L.S. (2010). Mapping the architecture of the HIV-1 Tat circuit: A decision-making circuit that lacks bistability and exploits stochastic noise. Methods <doi:10.1016/j.ymeth.2010.12.006> PMID 21167940

Walston T, and Hardin J. (2010). Acquisition of 4D DIC Microscopic Data to Determine Cell Contacts in Caenorhabditis elegans Embryos. Cold Spring Harb Protoc. 2010:pdb.prot5541. doi: 10.1101/pdb.prot5541 PMID 21123428

Uehara R, and Goshima, G. (2010). Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase. JCB 191:259-267. <doi:10.1083/jcb.201004150> PMID 20937700 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Moss, J, et al. (2010). Break-induced ATR and Ddb1–Cul4Cdt2 ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast. Genes & Dev. 24: 2705-2716 doi: 10.1101/gad.1970810 PMID 21123655 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Brian Alan Ashcroft and Tjerk Oosterkamp (2010). AutoMicromanager: A microscopy scripting toolkit for LABVIEW and other programming environments. Rev. Sci. Instrum. 81:113708-113712 <doi:10.1063/1.3499370> PMID 21133479

Goldsmith AD, Sarin S, Lockery S, and Hobert, O. (2010) Developmental control of lateralized neuron size in the nematode Caenorhabditis elegans. Neural Development 5:33 <doi:10.1186/1749-8104-5-33> PMID 21122110 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Biehlmaier O, Hehl J, and Csucs G. (2010). Acquisition speed comparison of microscope software programs. Microsc Res Tech. (Epub ahead of print) DOI: 10.1002/jemt.20944 PMID 21086451

Liisa M. Hirvonen, and Trevor A. Smith. (2010). Imaging on the Nanoscale: Super-Resolution Fluorescence Microscopy Australian Journal of Chemistry 64: 415-45 <doi:10.1071/CH10333>

Yehonathan Bar-Yosef, Assaf Sukenik, Ora Hadas, Yehudit Viner-Mozzini and Aaron Kaplan (2010). Enslavement in the Water Body by Toxic Aphanizomenon ovalisporum, Inducing Alkaline Phosphatase in Phytoplanktons. Current Biology 20:1557-1561. <doi:10.1016/j.cub.2010.07.032> PMID 20705465

Scott D. Hansen and R. Dyche Mullins (2010). VASP is a processive actin polymerase that requires monomeric actin for barbed end association. JCB 191:571-584. <doi:10.1083/jcb.201003014> PMID 21041447 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Nicholas W. Frost and Michael T. Bowser (2010). Using buffer additives to improve analyte stream stability in micro free flow electrophoresis. Lab Chip 10:1231-1236 doi: 10.1039/B922325H PMID 20445874 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Guangshuo Ou, Nico Stuurman, Michael D’Ambrosio, and Ronald D. Vale (2010). Polarized Myosin Produces Unequal-Size Daughters During Asymmetric Cell Division. Science 330:677-680. <doi:10.1126/science.1196112> PMID 20929735 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Benjamin S. Freedman, Kelly E. Miller, Rebecca Heald (2010). Xenopus Egg Extracts Increase Dynamics of Histone H1 on Sperm Chromatin. PLoS ONE 5(9): e13111. <doi:10.1371/journal.pone.0013111> PMID 20927327 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Simon Blanchoud, Yemima Budirahardja, Félix Naef, Pierre Gönczy (2010). ASSET: A robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos. Developmental Dynamics 239:3285-3296. <doi:10.1002/dvdy.22486> PMID 21089077

Sarah S. Goodwin and Ronald D. Vale (2010.) Patronin Regulates the Microtubule Network by Protecting Microtubule Minus Ends. Cell 143:263-274. <doi:10.1016/j.cell.2010.09.022> PMID 20946984 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Michael V. D’Ambrosio and Ronald D. Vale (2010). A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. J Cell Biol 191:471-478. <doi:10.1083/jcb.201003135> PMID 21041442 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Veliz LA, Toro CA, Vivar JP, Arias LA, Villegas J, et al. (2010). Near-Membrane Dynamics and Capture of TRPM8 Channels within Transient Confinement Domains. PLoS ONE 5(10): e13290. <doi:10.1371/journal.pone.0013290> PMID 20948964 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Douglas R. Mackay, Katharine S. Ullman, Christopher K. Rodesch (2010). Time-lapse Imaging of Mitosis After siRNA Transfection (2010) J Vis Exp. 40. doi: 10.3791/1878 PMID 20548280

Zhucheng Chen, Dominika Borek, Shae B. Padrick, Timothy S. Gomez, Zoltan Metlagel, Ayman M. Ismail, Junko Umetani, Daniel D. Billadeau, Zbyszek Otwinowski, and Michael K. Rosen (2010). Structure and control of the actin regulatory WAVE complex. Nature 468:533–538. <doi:10.1038/nature09623> PMID 21107423 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

C. M. Perrault, M. A. Qasaimeh, T. Brastaviceanu, K. Anderson, Y. Kabakibo, and D. Juncker (2010). Integrated microfluidic probe station. Rev. Sci. Instrum. 81:115107-115115. <doi:10.1063/1.3497302> PMID 21133501

Arthur Edelstein, Nenad Amodaj, Karl Hoover, Ron Vale, and Nico Stuurman (2010) Computer Control of Microscopes Using μManager. Current Protocols in Molecular Biology 14.20.1-14.20.17 <doi:10.1002/0471142727.mb1420s92> PMID 20890901 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Erin D. Goley, Aravind Rammohan, Elizabeth A. Znameroski, Elif Nur Firat-Karalar, David Sept, and Matthew D. Welch (2010). An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. PNAS 107:8159-8164. doi: 10.1073/pnas.0911668107 PMID 20404198 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Yu C-h, Wu H-J, Kaizuka Y, Vale RD, Groves JT (2010) Altered Actin Centripetal Retrograde Flow in Physically Restricted Immunological Synapses. PLoS ONE 5(7): e11878. <doi:10.1371/journal.pone.0011878> PMID 20686692 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Karin G. Stenkula, Vladimir A. Lizunov, 3, Samuel W. Cushman and Joshua Zimmerberg (2010). Insulin Controls the Spatial Distribution of GLUT4 on the Cell Surface through Regulation of Its Postfusion Dispersal. Cell Metabolism 12:250-259 <doi:10.1016/j.cmet.2010.08.005> PMID 20816091

Michael J. Boyle, Rachael L. French, K. Amber Cosand, Jennie B. Dorman, Daniel P. Kiehart and Celeste A. Berg (2010). Division of labor: Subsets of dorsal-appendage-forming cells control the shape of the entire tube. Developmental Biology 346:68-79 <doi::10.1016/j.ydbio.2010.07.018> PMID 20659448

Kurt Thorn (2010). Spinning-Disk Confocal Microscopy of Yeast. Methods in Enzymology 470:581-602 <doi::10.1016/S0076-6879(10)70023-9> PMID 20946826

Shalin B. Mehta and Colin J. R. Sheppard (2010). Sample-less calibration of the differential interference contrast microscope. Applied optics 49: 2954-2968 <doi:10.1364/AO.49.002954> PMID 20490258

Ricardo Henriques, Mickael Lelek, Eugenio F Fornasiero, Flavia Valtorta, Christophe Zimmer & Musa M Mhlanga (2010). QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nature Methods 7:339–340 <doi:10.1038/nmeth0510-339> PMID 20431545

Biswadip Das, Melanie N. Cash, Arthur R. Hand, Armin Shivazad, Scott S. Grieshaber, Bently Robinson and David J. Culp (2010) Tissue Distribution of Murine Muc19/Smgc Gene Products. Journal of Histochemistry & Cytochemistry 58(2): 141-156 <doi:10.1369/jhc.2009.954891> PMID 19826070 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

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Shan Shan Kou, Laura Waller, George Barbastathis, and Colin J. R. Sheppard (2010) Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. Optics Letters 35(3):447-449 <doi:10.1364/OL.35.000447> PMID 20125750

John Oreopoulos, Raquel F. Epand, Richard M. Epand, and Christopher M. Yip (2010) Peptide-induced domain formation in supported lipid bilayers: Direct evidence by combined atomic force and polarized total internal reflection fluorescence microscopy. Biophysical Journal 98(5):815-823 <doi:10.1016/j.bpj.2009.12.486> PMID 20197035 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Christian Conrad and Daniel Gerlich (2010). Automated microscopy for high-content RNAi screening. Journal of Cell Biology 188/4:453-461 <doi:10.1083/jcb.200910105> PMID 20176920 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

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David K. Breslow, Sean R. Collins, Bernd Bodenmiller, Ruedi Aebersold, Kai Simons, Andrej Shevchenko, Christer S. Ejsing, and Jonathan S. Weissman (2010). Orm family proteins mediate sphingolipid homeostasis. Nature 463:1048-1053 <doi:10.1038/nature08787> PMID 20182505 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Total 2010 Citations: 50

2009

Thomas M Suchyna, Vladamir S Markin and Frederick Sachs (2009). Biophysics and Structure of the Patch and Gigaseal. Biophysical Journal 97: 738-747 <doi:10.1016/j.bpj.2009.05.018> PMID 19651032 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] 19749742

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] 19325107

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] 19556461

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}] 19948500

Matula, P., Maška, M., Danek, O., Matula, P., and Kozubek, M. (2009). Acquiarium: free software for the acquisition and analysis of 3D images of cells in fluorescence microscopy. In Proceedings of the Sixth IEEE international Conference on Symposium on Biomedical Imaging: From Nano To Macro (Boston, Massachusetts, USA, June 28 - July 01, 2009). IEEE Press, Piscataway, NJ, 1138-1141 7

Jungho Ohn, Huai-Jen Tsai, and Michael Liebling (2009). Joint dynamic imaging of morphogenesis and function in the developing heart. Organogenesis 5:166-173 link to pdf

A. Bernardini, C. Wotzlaw, H.-G. Lipinski, and J. Fandrey (2009). An Automated Real Time System for FRET Based Protein-Protein Interaction Analysis. IFMBE Proceedings 25/4:1056-1058 <doi:10.1007/978-3-642-03882-2_281>

James C. W. Locke and Michael B. Elowitz (2009). Using movies to analyse gene circuit dynamics in single cells. Nature Reviews Microbiology. <doi:10.1038/nrmicro2056>. PMID

1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Vedakumar Tatavarty, Eun-Ji Kim, Vladimir Rodionov, and Ji Yu (2009). Investigating Sub-Spine Actin Dynamics in Rat Hippocampal Neurons with Super-Resolution Optical Imaging. PLoS One. <doi:10.1371/journal.pone.0007724>. PMID 19898630. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Biswadip Das, Melanie N. Cash, Arthur R. Hand, Armin Shivazad, Scott S. Grieshaber, Bently Robinson, and David J. Culp (2009). Tissue Distibution of Murine Muc19/Smgc Gene Products. J. Histochem. and Cytochem. <doi:10.1369/jhc.2009.954891>. PMID 19826070. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Ronald D. Vale, James A. Spudich, and Eric R. Griffis (2009). Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. J. Cell Biol. 186:727-738 <doi:10.1083/jcb.200902083>. PMID 19720876. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Grossmann S, Higashiyama S, Oksche A, Schaefer M, and Tannert A (2009). Localisation of endothelin B receptor variants to plasma membrane microdomains and its effects on downstream signalling. Mol Membr Biol. 26:279-292. <doi:10.1080/09687680903191682>. PMID 19757321.

Miao-Hsueh Chen, Christopher W. Wilson, Ya-Jun Li, Kelvin King Lo Law, Chi-Sheng Lu, Rhodora Gacayan, Xiaoyun Zhang, Chi-chung Hui, and Pao-Tien Chuang (2009). Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved. Genes & Development 23:1910-1928 doi: 10.1101/gad.1794109. PMID

1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Christopher W. Wilson, Catherine T. Nguyen, Miao-Hsueh Chen, Jehn-Hsiahn Yang, Rhodora Gacayan, Jie Huang, Jau-Nian Chen & Pao-Tien Chuang (2009). Fused has evolved divergent roles in vertebrate Hedgehog signalling and motile ciliogenesis. Nature 459:98-102 <doi:10.1038/nature07883>

Margaret Lin Veruki and Espen Hartveit (2009). Meclofenamic Acid Blocks Electrical Synapses of Retinal AII Amacrine and ON-Cone Bipolar Cells. ‘'’J Neurophysiol ‘’’ 101: 2339-2347 <doi:10.1152/jn.00112.2009>. PMID 19279153.

Jonathan M. Glynn, Yue Yang, Stanislav Vitha, Aaron J. Schmitz, Mia Hemmes, Shin-ya Miyagishima, and Katherine W. Osteryoung (2009). PARC6, a novel chloroplast division factor, influences FtsZ assembly and is required for recruitment of PDV1 during chloroplast division in Arabidopsis. The Plant Journal 59:700-711 <doi:10.1111/j.1365-313X.2009.03905.x>. PMID 19453460.

Yoshihisa Kaizuka, Adam D. Douglass, Santosh Vardhana, Michael L. Dustin, and Ronald D. Vale (2009). The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. J. Cell Biol. [<doi:10.1083/jcb.200809136>. PMID 19398758. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Guangshuo Ou, and Ronald D. Vale (2009). Molecular signatures of cell migration in C. elegans Q neuroblasts. J. Cell Biol. 185:77-85 <doi:10.1083/jcb.200812077>. PMID 19349580. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Liisa M. Hirvonen, Kai Wicker, Ondrej Mandula and Rainer Heintzmann (2009). Structured illumination microscopy of a living cell. European Biophysics Journal 38:807-812 <doi:10.1007/s00249-009-0501-6>. PMID 19536533.

J. Bradley Zuchero, Amanda S. Coutts, Margot E. Quinlan, Nicholas B. La Thangue & R. Dyche Mullins (2009). p53-cofactor JMY is a multifunctional actin nucleation factor. Nature Cell Biology 11:451-459 <doi:10.1038/ncb1852>. PMID

1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Andrew D Straw, and Michael H Dickinson (2009). Motmot, an open-source toolkit for realtime video acquisition and analysis. Source Code Biol Med. 4:5. <doi:10.1186/1751-0473-4-5>. PMID 19624853. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

H. Khodja, M. Hanot, b, M. Carrièr, b, J. Hoarau, b and J.F. Angulo (2009). The single-particle microbeam facility at CEA-Saclay. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 267:1999-2002. <doi:10.1016/j.nimb.2009.03.040>

Franco J. Vizeacoumar, Yolanda Chong, Charles Boone and Brenda J. Andrews (2009). A picture is worth a thousand words: Genomics to phenomics in the yeast Saccharomyces cerevisiae. FEBS Letters. 583:1656-1661. <doi:10.1016/j.febslet.2009.03.068> PMID 19351535.

X. Michalet, R.A. Colyer, J. Antelman, O.H.W. Siegmund, A. Tremsin, J.V. Vallerga, and S. Weiss (2009). Single-Quantum Dot Imaging with a Photon Counting Camera. Current Pharmaceutical Biotechnology 10:543-558 PMID 19689323 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

John Oreopoulos and Christopher M. Yip (2009). Combinatorial microscopy for the study of protein–membrane interactions in supported lipid bilayers: Order parameter measurements by combined polarized TIRFM/AFM. J Struct Biol. 168:21-36. <doi:10.1016/j.jsb.2009.02.011>. PMID 19268707.

Julia R. Kardon, Samara L. Reck-Peterson, and Ronald D. Vale (2009). Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin. PNAS. 106:5669-5674. doi: 10.1073/pnas.0900976106 PMID

1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Samways, D.S., Harkins, A.B. & Egan, T.M (2009). Native and recombinant ASIC1a receptors conduct negligible Ca2+ entry. Cell Calcium. 45:319-325. <doi:10.1016/j.ceca.2008.12.002>. PMID 19185346. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Chambers, R.D. & Santiago, J.G (2009). Imaging and Quantification of Isotachophoresis Zones Using Nonfocusing Fluorescent Tracers. Analytical Chemistry. Early publication online. <doi:10.1021/ac802698a>. PMID 19290665.

Total 2009 Citations: 28

2008

content<h1 id="pre-2021-documentation-for-photometrics-pvcam-device-adapter">Pre-2021 documentation for Photometrics PVCAM Device Adapter</h1>

Summary:	Interfaces with Roper, Photometrics (this adapter used to work with Princeton Instruments cameras, however, now please use the Princeton Instruments adapter)
Authors:	Nenad Amodaj, with contributions from Nico Stuurman (Mac adaptation) and Ji Yu (expose more PVCAM properties), Oleksiy Danikhnov and Arthur Edelstein (reliability in streaming mode).
License:	BSD
Platforms:	Windows and Mac OS X (linux possible but not tested), for Windows 7 see issue below
Devices:	PVCAM compatible cameras
Example Config File:	MMConfig_pvcam_simple.cfg
Driver Needed:	Roper/Photometrics PVCAM

Installation

Make sure you have the latest version of the Photometrics PVCAM Driver supporting your camera. You might need to update PVCAM after you upgrade Micro-Manager (if you get an error, of PVCAM becomes “unavailable”).

Photometrics

This adapter should work with Photometrics PVCAM compatible cameras that work with recent PVCAM versions (If you have an older Photometrics camera that does not work with a recent PVCAM version, please try the Princeton Instruments adapter). To use it, you will need to have the (PVCAM software) installed and working. At the very least, the pvcam test application (included with PVCAM) should give you an image, and preferably RSImage works with your camera (although we have seen cases where Micro-Manager works with a PVCAM camera whereas RSImage does not). We tested it with an old MicroMax, a newer Firewire CoolSnap, Cascade II, and Evolve cameras on Windows and a Firewire Coolsnap ES, EZ, and HQ2 on Intel Mac. Thanks to Ji Yu, most available camera properties are exposed.

The Photometrics website has a video showing how to configure Micro-Manager for use with a Photometrics camera.

Firewire issues

From the Photometrics website:

In recent updates, Microsoft released two hotfixes which updated their FireWire stack file. However, these updates cause performance drops in the functionality of Texas Instruments (TI) FireWire Controllers which ship with Photometrics cameras. These performance drops

manifest with the following symptoms:

• Image Tearing
• Interference Lines
• Black Images
• Reduced Frames-per-second

Installing the Thesycon 1394 stack fixes these problems by replacing the default Microsoft stack. This remedies any problems caused by the updates. The Thesycon FireWire stack has been thoroughly tested and does not cause any other conflicts or problems with any devices.

The Thesycon stack installer can be downloaded here.

Windows 7 issue

Paul Forscher reported that Micro-Manager does not find any PVCAM cameras under Windows 7. The problem is that Micro-Manager can not find the file ‘PVCAM.ini’. To fix this, run RSConfig.exe from the Photometrics folder in the start menu. This will create the proper PVCam. Ini in the c:\windows folder. If that fails, type ‘cmd’ in the “Search programs and files’ box in the Start menu, and then type:

copy C:\Users\username\AppData\Local\VirtualStore\Windows\PVCAM.ini C:\Program Files\Micro-Manager1.3

where you should replace ‘username’ with your username.

--Nico 11:33, 31 August 2007 (PDT)

Notable changes

Apr 8, 2016

Added support for Photometrics Prime camera. PrimeEnhance (DENOISING PP) and PrimeLocate (Centroids) features are supported. Users are kindly requested to upgrade PVCAM Runtime to version 3.1.9.1 that is available at www.photometrics.com. Customers who are using the Presets feature of Micro-Manager may need to re-create or update their preset configuration because names of some camera properties have changed. E.g. “Frame Transfer Parallel Clocking” has been shortened to “Frame Transfer”. A configuration that was prepared with older PVCAM and that is using such property will not work with the new PVCAM.

Feb 14, 2014

[Windows only] Added support for the new OptiMos camera. New property “ExposeOutMode” has been added. This property controls the expose out signal of the sCMOS sensor. Please see the OptiMos user manual for more details. This feature is supported since PVCAM 3.0.1.

Sep 5, 2013

[Windows only] Added support for FRAME_INFO metadata and callback acquisition. The FRAME_INFO provides precise frame time-stamps with supported cameras. Users are kindly requested to upgrade PVCAM to version 2.9.11.

Jun 20, 2013

The adapter now supports micro-second exposure resolution. Exposure values shorter than 60ms are automatically treated in micro-second resolution. From now users can use exposure values like 50.5, 2.8, 0.09, etc.

Click here to search for PVCAM on the Micro-Manager mailing list archive.

} }, }. (}). }. ‘’’}.’’’ }:}. [http://dx.doi.org/} doi:}]

Moreira KE, Walther TC, Aguilar PS, Walter P. (2008) Pil1 Controls Eisosome Biogenesis. Mol. Biol. Cell. 20: 809-818. <doi:10.1091/mbc.E08-03-0313>. PMID 19037108. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Olivier Debeir, Ivan Adanja, Robert Kiss & Christine Decaestecker (2008). Models of cancer cell migration and cellular imaging and analysis. Chapter 5 of The motile actin system in health and disease. Edited by: Lambrechts A, Ampe C. Kerala: Transworld Research Network, pp 123-156. PDF

Bashkirov, P.V. et al (2008). GTPase Cycle of Dynamin Is Coupled to Membrane Squeeze and Release, Leading to Spontaneous Fission. Cell. 135:1276-1286. <doi:10.1016/j.cell.2008.11.028>. PMID 19084269. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Zimberlin, J.A., Wadsworth, P. & Crosby, A.J . Living microlens arrays (2008). Cell Motility and the Cytoskeleton. 65. <doi:10.1002/cm.20302>. PMID

1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Aragón, T. et al (2008). Messenger RNA targeting to endoplasmic reticulum stress signalling sites. Nature. 457:736-740. <doi:10.1038/nature07641>. PMID

1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Merksamer, P.I., Trusina, A. & Papa, F.R (2008). Real-Time Redox Measurements during Endoplasmic Reticulum Stress Reveal Interlinked Protein Folding Functions. Cell. 135:933-947. <doi:10.1016/j.cell.2008.10.011>. PMID 19026441. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Chernomoretz, A. et al (2008). Using Cell-ID 1.4 with R for microscope-based cytometry. Current protocols in molecular biology. Unit 14.18. <doi:10.1002/0471142727.mb1418s84>. PMID 18972382. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Ingrid C. Koo, Chen Wang, Sridharan Raghavan, J. Hiroshi Morisaki, Jeffery S. Cox and Eric J. Brown (2008). ESX-1-dependent cytolysis in lysosome secretion and inflammasome activation during mycobacterial infection. Cellular Microbiology. 10(9):1866-1878 <doi:10.1111/j.1462-5822.2008.01177.x>. PMID 18503637. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Erika Rosivatz (2008). Imaging the boundaries—innovative tools for microscopy of living cells and real-time imaging. ’ J Chem. Biol.’ <doi:10.1007/s12154-008-0004-4>. PMID 19568794. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Lili Niu and Ji Yu (2008). Investigating Intracellular Dynamics of FtsZ Cytoskeleton with Photo-activation Single-molecule Tracking. Biophysical J. 95:2009-2016 <doi:10.1529/biophysj.108.128751>. PMID 18390602. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Liam J. Holt, Andrew N. Krutchinsky, and David O. Morgan (2008). Positive feedback sharpens the anaphase switch. Nature 454:353-357 PMID 18552837 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

G. Goshima, M. Mayer, N. Zhang, N. Stuurman, and R.D. Vale (2008). Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J Cell Biol. 181(3):421-429 PMID 18443220 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Griffis E.R., Stuurman N., Vale R.D. (2008). Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol. 177(6):1005-1015 PMID 17576797 [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Total 2008 Citations: 14

2007

Christopher S. Campbell and R. Dyche Mullins (2007). In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids. J Cell biol. 179(5):1059-1066 <doi:10.1083/jcb.200708206>. PMID 18039937. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Yi Yang, Amira M. El-Ganiny, Geoffrey E. Bray, David A.R. Sanders, and Susan G.W. Kaminskyj (2007). Aspergillus nidulans hypB encodes a Sec7-domain protein important for hyphal morphogenesis. Fungal Genetics and Biology 45(5):749-759. <doi:10.1016/j.fgb.2007.11.005>. PMID 18248749.

Stuurman, N., Amodaj N., Vale, R.D. (2007). Micro-Manager: Open Source software for light microscope imaging. Microscopy Today. 15(3):42-43. Full Issue (pdf)

Kevin C. Slep and Ronald D. Vale (2007). Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170, and EB1. Molecular Cell 27(6):976-991. <doi:10.1016/j.molcel.2007.07.023>. PMID 17889670. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Liam J. Holt, Jessica E. Hutti, Lewis C. Cantley and David O. Morgan (2007). Evolution of Ime2 Phosphorylation Sites on Cdk1 Substrates Provides a Mechanism to Limit the Effects of the Phosphatase Cdc14 in Meiosis. Molecular Cell 25(5):689-702. <doi:10.1016/j.molcel.2007.02.012>. PMID 17349956. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Tony J. Collins. (2007) ImageJ for microscopy. BioTechniques 43:S25-S30 doi 10.2144/000112517. PMID 112517.

Carpenter, A.E. (2007). Image-based chemical screening. Nature Chemical Biology. 3:461-465. <doi:10.1038/nchembio.2007.15>. PMID 17637778.

Carpenter, A.E. (2007). Software opens the door to quantitative imaging. Nature Methods. 4:120-121. PMID 17264858.

Gordon A., Colman-Lerner A., Chin T.E., Benjamin K.R., Yu R.C., Brent R. (2007) Single-cell quantification of molecules and rates using open-source microscope-based cytometry. Nature Methods. 4:175-181. PMID 17237792.

Goshima G., Wollman R., Goodwin S.S., Zhang N., Scholey J.M., Vale R.D., Stuurman N. (2007). Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316:417-421. PMID 17412918. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Garner E.C., Campbell C.S., Weibel D.B., Mullins R.D. (2007). Reconstitution of DNA segregation driven by assemebly of a prokaryotic actin homolog. Science. 315:1270-1274. PMID 17332412. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid={include.text}]

Total 2007 Citations: 11